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KMID : 0385520140270060277
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Analytical Science & Technology
2014 Volume.27 No. 6 p.277 ~ p.283
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Effect of concentration, pressure, and cut-off size on removing phospholipase A2 in bee venom by ultrafiltration
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Lee Ji-yeon
Kim Choul-Goo
Kim Il-Kwang
Lee Jong-Soo
Kim Eui-kyung
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Abstract
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The effects of concentration, pressure, and molecular sige on removing allergenic substance (phospholipase A2) in bee venom by ultrafiltration were investigated. The membrane pore sizes were selected based on the molecular weight of the main compounds. The conditions of concentration and pressure were selected randomly. As results, we obtained the optimum condition (1 mg/mL, 20 psi, 10,000 dalton) for removing PLA2 at constant concentration of melittin and apamin and confirmed the separation results by HPLC and SDS-PAGE.
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KEYWORD
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honey, bee venom, phospholipase A2, ultrafiltration,
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